qdoI [2019-08-13 09:59:54]

Fe-containing quercetin 2,3-dioxygenase

locus: BSU_39980

pI: 5.56

mw: 37.43 kDa

protein length: 337 aa

gene length: 1014 bp

function: resistance to plant product quercetin

product: Fe-containing quercetin 2,3-dioxygenase

essential: no

synonyms: yxaG

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 4,106,245 4,107,258

The protein

Catalyzed reaction/ biological activity

• oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO

Protein family

• bicupin family

[SW|Domains]

• 2 [SW|cupin 2 domain]s (aa 55 ... 110, aa 226 ... 281)

[SW|Cofactors]

• Mn(II) (preferred), but also active with Fe(II) snd Co(II) [Pubmed|22084064]

Structure

• [PDB|1Y3T] [Pubmed|15628860]
• [PDB|2H0V]

Expression and Regulation

Operons

• genes: [gene|6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A|qdoI]-[gene|142444E1167BA7428EB95E4418C839ADF3E728B0|yxaH]
  description: [Pubmed|10746760]

  regulatory mechanism

  • [protein|52D560AA02F0849CB24460A496021560063B2E12|LmrA]: repression, [Pubmed|15317768], in [regulon|52D560AA02F0849CB24460A496021560063B2E12|LmrA regulon]
  • [protein|47387014B5E89BF218BA94D8C2A570479B1EFD4E|QdoR]: repression, [Pubmed|17483215], in [regulon|47387014B5E89BF218BA94D8C2A570479B1EFD4E|QdoR regulon]

  regulation

  • induced by flavonoids such as quercetin ([protein|search|QdoR], [protein|search|LmrA]) [Pubmed|17483215]

  additional information

  • major regulator: [protein|search|QdoR] [PubMed|17483215]

Biological materials

Mutant

• MGNA-B684 (yxaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1683 NBRP B. subtilis, Japan]
• BKE39980 ([gene|6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A|qdoI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCGCTTCCTCCAA, downstream forward: _UP4_TAAAGATGACAAGATTGTAA
• BKK39980 ([gene|6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A|qdoI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCGCTTCCTCCAA, downstream forward: _UP4_TAAAGATGACAAGATTGTAA

References

Reviews

• 9056848,10730195

Original publications

• 17483215,14741339,15039076,15317768,10746760,20460727,14697267,22084064,16411777,27170159,15628860